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. 2026 Jan 23;16:6066. doi: 10.1038/s41598-026-37189-4

Table 2.

Crystallization conditions and electron density features of TPST2 structures.

TPST2Na (PDB: 9WWE) TPST2Mn (PDB: 9WWF) TPST2pep (PDB: 3AP1) TPST2apo (PDB: 3AP3) Co (ano) Zn (ano) Cu (ano)
Cation concentration (mM)
Na 120 120 300 200 120 120 120
K 100 100 - - 100 100 100
Zn 25 1 - - 1 10 1
Mn - 20 1 1 - - -
Co - - - - 10 - -
Cu - - - - - - 10
Peak height (2FoFc / anomalous; σ)
Metal binding site 1 8.1 / NA 5.8 / 8.5 2.6 / - - / - 5.1 / 5.2 4.3 / - 3.7 / -
Metal binding site 2 10.1 / NA 7.6 / 5.8 5.2 / - - / - 4.5 / 3.5 4.3 / - 3.4 / -
Crystallographic lattice site 1 28.5 / NA 23.3 / 10.7 - / - - / - 16.8 / 5 13.3 / 46.0 14.6 / -
Crystallographic lattice site 2 20.3 / NA - / - - / - - / - - / - 4.2 / 9.3 - / -

Values indicate crystallization cation concentrations and corresponding 2Fo-Fc / anomalous peak heights (σ) observed at metal-binding or lattice sites.