Table I. Percentage crosslinking yields (obtained with internally labeled s4U*GA mRNA analog) and RF activity of Met mutants relative to wild-type eRF1.
| Wild type | S60M | I62M | K63M | S64M | V66M | G73M | |
|---|---|---|---|---|---|---|---|
| Crosslink |
100 |
97 |
95 |
43 |
85 |
98 |
80 |
| RF activity with UAA |
100 |
100 |
7 |
61 |
100 |
52 |
nd |
| RF activity with UAG |
100 |
100 |
5 |
67 |
100 |
30 |
nd |
| RF activity with UGA | 100 | 100 | 28 | 66 | 100 | 39 | nd |
Values are the averages of at least three independent experiments and are given with ±10% (crosslinks) and ±11% (RF activity) precision.
nd, not determined.