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Fig. 6. IEX-1 expression is involved in the long-lasting ERK activation induced by TPO in UT7 cells. UT7 cells were electroporated with plasmids encoding HA-ERK1 (upper panel) or HA-IEX-1 (bottom panel) together with either IEX-1 or GFP siRNA duplexes, as indicated. The activity of HA-ERK was assessed in anti-HA-immunoprecipitates following various times of TPO stimulation.
