Fig. 8. IEX-1 inhibits cell death induced by various stimuli. (A and B) Clones of UT7 cells stably transfected with empty (Neo) or HA-IEX-1 (Wt) encoding plasmids were cultured with EPO. Apoptosis was induced by removing EPO from the culture medium and measured by annexin V–FITC staining (A) or immunoblotting with anti-caspase-3 antibodies (B). Anti-HA immunoblots show expression of the transgene in the different clones (lower panels). (C) CHO or (D) HeLa cells were transiently transfected with pEGFP or pEGFP-IEX-1. Twenty-four hours post-transfection, the cells were treated with STS (C) or TNF + CHX (D), as indicated. Apoptosis of the EGFP-positive transfected cells was determined as described in Materials and methods. (D) Mean ± SE of three independent transfections.