Fig. 6. Localization of CENP-H and -C to the Xp-side of the DXZ1 array using immuno-FISH. (A and A′) Chromatin fibres from 1aH2, a DT40 line carrying the IKNFA3 minichromosome and producing a CENPH–GFP fusion protein, were generated. The centromerically located fusion protein was detected (on all chromosomes in the cell) using anti-GFP (green). This was followed by dual FISH for the human X centromere-based minichromosome using DXZ1 (red) and BAC 342O19 (which spans the γ-satellite array DNA) (white). (B and B′) Chromatin fibres from the DT40 cell line IKNFA3. CENP-C has been detected using antibodies to chicken CENP-C (green) followed by dual FISH for DXZ1 (red) and BAC342O19 (white).