Fig. 7. Transgenic expression of TIAR during SeV infection. Parallel HeLa cell cultures were infected with 10 infectious units of each of the six rSeV indicated (see text), or mock infected, and harvested at 48 h.p.i. Half of the cells were used to determine the intracellular levels of the transgenes and the SeV N protein by immunoblotting with anti-HA, anti-GFP and anti-N (A). The other half was used to determine the level of annexin V staining by FACS (C). For the three rSeV that express GFP, the level of intracellular replication was determined more accurately by following GFP fluorescence directly by FACS (B). The error bars in (C) indicate the results of infecting duplicate cultures.