Table I. Partial list of the proteins displaying >2-fold expression differences.
| Category | Protein namea | L [Myc(–)]: H [Myc(+)] ratiob | nb | Notes | Reference |
|---|---|---|---|---|---|
| Adhesion | CD166/HDL-R | L > H | |||
| ICAM-1 | 1:0.21 | 1 | |||
| N-CAM 140 | 1:0.25 | 1 | Guo et al. (2000) | ||
| integrinβ1 | 1:0.40 ± 0.07 | 4 | |||
| PINCH | 1:0.37 ± 0.10 | 3 | Integrin signaling | Guo et al. (2000) | |
| ESL-1 | 1:0.37 ± 0.09 | 16 | E-selectin ligand 1 | ||
| LAMP1 | 1:0.45 | 1 | Presents carbohydrate ligand to selectin | Guo et al. (2000) | |
| Rho pathway | Rho A | 1:0.49 ± 0.04 | 5 | Induces the formation of actin stress fibers and focal adhesions | |
| CDC42 | 1:0.48 ± 0.04 | 2 | Induces the formation of actin stress fibers and focal adhesions | ||
| Rok α | 1:0.23 ± 0.11 | 3 | Induces the formation of actin stress fibers and focal adhesions | ||
| Actin network | α-actin | 1:0.21 ± 0.04 | 3 | ||
| β-actin | 1:0.33 ± 0.06 | 11 | |||
| cofilin | 1:0.48 ± 0.08 | 11 | Actin binding | ||
| profilin | 1:0.46 ± 0.07 | 9 | Actin binding | ||
| calponin | 1:0.24 | 1 | Actin/TPM/CaM binding | ||
| transgelin2 | 1:0.22 ± 0.06 | 7 | Actin crosslinking/calponin family | ||
| t-plastin | 1:0.21 ± 0.10 | 3 | Actin bundling | ||
| sid23p | 1:0.49 ± 0.03 | 9 | Actin depolymerization factor | ||
| f-actin capping protein β | 1:0.26 ± 0.05 | 8 | |||
| Eplin α | 1:0.30 | 1 | Epithelial protein lost in neoplasia, localizes to filamentous actin and suppresses proliferation when overexpressed | ||
| ABP-280 | 1:0.18 ± 0.02 | 2 | Endothelial actin binding protein | ||
| α-actinin 1 | 1:0.34 ± 0.09 | 11 | Actin binding | ||
| α-actinin 4 | 1:0.40 ± 0.08 | 3 | Actin binding | ||
| α-1 catenin | 1:0.43 ± 0.03 | 2 | |||
| β catenin | 1:0.39 ± 0.11 | 4 | |||
| talin | 1:0.38 ± 0.08 | 16 | Involved in connections of major cytoskeletal structures to the plasma membrane | ||
| Protein synthesis | 40S/60S ribosomal proteins (20 subunits) | 1:2–3 | Coller et al. (2000); Guo et al. (2000); Boon et al. (2001); Neiman et al. (2001); Schuhmacher et al. (2001) | ||
| eIF2B δ | 1:2.01 ± 0.26 | 2 | Translation | ||
| DEAD box RNA helicase P47 | 1:3.23 ± 0.10 | 2 | RNA helicase | ||
| DDX5 | 1:2.11 ± 0.03 | 8 | RNA helicase | ||
| DDX17 | 1:2.56 ± 0.00 | 2 | RNA helicase | ||
| DDX21 | 1:5.56 ± 0.00 | 2 | RNA helicase | ||
| fibrillarin | 1:3.64 ± 0.70 | 2 | Ribosome biogenesis | Coller et al. (2000) | |
| Nop56 | 1:2.56 | 1 | rRNA processing and assembly | ||
| Nop58 | 1:4.03 ± 0.58 | 3 | rRNA processing and assembly | ||
| Bop1 (block of proliferation 1) | 1:3.13 | 1 | rRNA processing and assembly | ||
| Protein degradation | Asn endopeptidose (legumain) | 1:0.27 ± 0.00 | 3 | ||
| aminopeptidase PILS | L > H | ||||
| Niban (calpain-like) | 1:0.20 | 1 | |||
| calpain 2 | 1:0.42 ± 0.07 | 2 | |||
| calpain regulatory subunit | 1:0.31 ± 0.00 | 2 | |||
| cathepsin B | 1:0.27 ± 0.06 | 7 | |||
| cathepsin D | 1:0.26 ± 0.00 | 2 | |||
| cathepsin L | 1:0.26 | 1 | |||
| endooligopeptidase | 1:0.29 ± 0.05 | 2 | |||
| prothrombin | L > H | ||||
| Metabolism | Fatty acid synthase | 1:2.99 ± 0.62 | 6 | Schuhmacher et al. (2001) | |
| adenylate kinase 2 | 1:2.00 | 1 | AMP + ATP → 2ADP | ||
| cad* | 1:2.13 | 1 | First, second and third steps of pyrimidine biosynthesis | Schuhmacher et al. (2001) | |
| amidophosphoribosyl transferase (Pur 1) | 1:9.10 | 1 | First step of purine biosynthesis | ||
| adenosine 3 | 1:2.06 ± 0.06 | 2 | Second, third and fifth steps of purine biosynthesis | ||
| Ade 2 (Pur 6) | 1:2.45 ± 1.29 | 13 | Sixth and seventh steps of purine biosynthesis | ||
| Ser hydroxymethyl transferase | 1:3.23 | 1 | Key enzyme in the biosynthesis of purines, lipids, hormones and other components | ||
| phosphoserine aminotransferase | 1:5.56 ± 0.00 | 2 | Biosynthesis of Ser and pyridoxine | ||
| Asn tRNA synthetase | 1:2.04 | 1 | |||
| ANT2 | 1:3.35 ± 0.67 | 4 | Mitochondrial ATP translocase | Guo et al. (2000) | |
| Other Myc targets | Ref-1/Ape | 1:6.11 ± 0.79 | 3 | Redox factor-1/apurinic-apyrimidic endonuclease | Guo et al. (2000) |
| MIF | 1:2.37 ± 0.29 | 5 | Macrophage migration inhibitory factor | Guo et al. (2000) | |
| hsp60* | 1:4.05 ± 1.13 | 9 | Coller et al. (2000); Guo et al. (2000); Boon et al. (2001); Neiman et al. (2001) | ||
| Tropomyosin α | 1:0.26 ± 0.10 | 3 | Coller et al. (2000); Guo et al. (2000) |
aPreviously proposed direct Myc targets are marked with an asterisk. The proteins that were also identified by the previous microarray analyses are in bold and the relevant references are cited.
bThe ratio of light ICAT™ reagent-labeled peptide (L) [derived from Myc(–) cells] and heavy ICAT™ reagent-labeled peptide (H) [derived from Myc(+) cells] is shown together with the standard deviation based on the number of independent peptides (n) identified and quantified. For proteins that displayed obvious expression differences, but were difficult to quantify, the ratio is marked L < H or L > H.
The complete list of proteins displaying >2-fold expression differences can be found in the Supplementary data.