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. 2025 Dec 23;136(4):e196536. doi: 10.1172/JCI196536

Figure 3. HIV RNA+ cells display heterogeneous phenotypes in the blood and colon of PWH.

Figure 3

(A) The frequency of cells with detectable viral RNA per million cells is displayed for each donor, separated by tissue of origin (blood/colon) and condition (unstimulated/PMAi/IL-2 stimulated). Horizontal bar represents mean values for each column. Each dot represents data from an individual donor. (B) Violin plot of normalized HIV expression level for each infected CD4+ T cell within the dataset divided by compartment. Blood cells are shown in red; colon cells are shown in blue. Each dot represents a single infected cell. (C) As in B, but with the data subdivided by stimulation condition. PMAi/IL-2 cells are shown in pale blue, and unstimulated cells are shown in purple. (D) UMAP visualization of the combined dataset with vRNA+ cells from the blood compartment highlighted in red. (E) As in D, but with vRNA+ cells from the colon compartment highlighted in blue. (F) Pie chart showing proportion of vRNA+ cells and total cells in each transcriptomic cluster for blood and colon cells separately. (G) Bar chart of the frequency of vRNA+ cells within each transcriptomic cluster for blood and colon tissue. Tem, effector memory T cell.