Fig. 5. Jafrac2 competes with Dronc for the binding of DIAP1. The ability of DIAP1 to bind to Dronc is significantly impaired in the presence of AKP- but not KP-Jafrac2. Co-purification of a catalytically inactive Dronc mutant (Dronc C>A) with DIAP1–GST in the presence of KP- or AKP-Jafrac2 from cellular extracts. Expression and purification of the indicated constructs was determined as in Figure 1D.