Fig. 7. Decreased collagen XVII shedding in TACE-deficient cells. (A) Murine keratinocytes from wild-type (WT) and TACE –/– mice were analyzed for collagen XVII shedding in vitro. Cells (C) and media (M) were sampled after 6 h and immunoblotted with a mixture of antibodies MO-NC16A and Endo-2 (left panel). The relative intensity of the signals of three individual experiments is expressed as a percentage ± SD (right panel). (B) Wild-type (WT) and TACE –/– fibroblasts were transfected with human collagen XVII cDNA. After 2 days the cells were analyzed for collagen XVII shedding. Cells (C) and media (M) were sampled after 6 h and immunoblotted with a mixture of antibodies MO-NC16A and Endo-2 (left panel). The relative intensity of the signals of two different experiments is expressed as a percentage ± SD (right panel).