Table 1.

PCR primers used to generate ITGB2 cDNA or genomic specific DNA fragments. Nucleotide numbering is according to position + 1 being the first nucleotide of the initiation codon ATG in the normal CD18 cDNA sequence. 5′UTR = 5′ untranslated region.

	Primer Sequence	Nucleotide position	Fragment Amplified	Size of the PCR Product
1	S: 5′CTACTCCATGCTTGATGACCTA 3′	402-423	CD18 extracellular	1.6 kb
	R: 5′CATCCACATAGATGAAGGTAGCG 3′	1973-1995	coding region
2	S: 5′GAGGAAATCGGCTGGCGAAC 3′	742-761	Exon 7	156 bp
	R: 5′ATGTTCGCTCGTTCCTTAAG 3′	877-896
3	S: 5′TGTGACACTGGCTACATTGGGA 3′	1412-1433	Exon 12	245 bp
	R: 5′AGACCTGGCCGTTGTAGCGC 3′	1636-1655
4	S: 5′GGAGGGGGCTCTGCTTCTG 3′	1658-1676	Exon 13	220 bp
	R: 5′ATGTACTTGCCACAGGGTGAG 3′	1858-1877
5	S: 5′CTCCTGCGCCGACTGCCT 3′	1879-1896	Exon 14	220 bp
	R: 5′CTCGGCTCTCATCCACATAG 3′	2079-2098
6	S: 5′ACAGAGTGCATCCAGGAGCA 3′	1252-1271	Exon 11	147 bp
	R: 5′CTCCAAGAAGCCCTTGCCAT 3′	1378-1397
7	S: 5′GAGTCCTTGCTCTGAAGATGACT 3′	−834,−812	CD18	1.1 kb
	R: 5′TGCTCTTGGTGGCAGGCACT 3′	230-249	5′UTR