Fig. 1. Amino acid starvation induces filamentous growth in C.albicans. (A) CAF-2 cells grown in YPD containing 10 mM 3AT or 10% serum for 2 h, stained with Calcofluor white, and visualized by light and fluorescence microscopy. Arrows highlight the positions of septa. (B) Percentage filament formation by C.albicans CAF2-1 (Table I) following 2 h growth at 37°C in YPD with the following additions: ± 20 mg/ml amino acids (open and closed bars, respectively); H2O control; 10 mM 3AT; 10% fetal calf serum; 10 mM dibutyryl cAMP.