Fig. 7. The GCN-like response in C.albicans is not dependent upon Efg1, Cph1 or Ras1. (A) Constitutive activation of the GCN-like response is not blocked by inactivation of Efg1, Cph1 or Ras1. Ten-fold dilutions of C.albicans cells, transformed with pACT1 (–) or pACT-GCN4 (+), were plated on synthetic complete medium ± 10 mM 3AT: WT, CAF2-1; gcn4, GTC43; cph1, JKC19; efg1, HLC52; cph1, egf1, HLC54; ras1, ras1-2/ras1-3 (Table I). (B) Constitutive activation of Ras signalling does not activate the GCN response in C.albicans. Northern blot analysis of CaARO4, CaLYS1 and CaGCN4 mRNA levels, relative to the 26S rRNA loading control, during the growth of RAS1/RAS1 (WT) or RAS1/RAS1/RAS1V13 cells (RASV13; Table I) on synthetic complete medium containing maltose ± 10 mM 3AT.