Fig. 1. Mesangial cell treatment protocol and ET-1 induction of hypertrophy. (A) ET-1 treatment protocol. The figure describes the time course of treatment for induction of hypertrophy. For certain experiments, as indicated in other figures, cells were harvested at earlier times in order to monitor regulation of signaling pathways or gene expression. (B) The 48 h treatment protocol in (A) is sufficient to trigger mesangial cell hypertrophy, an increase in [³H]leucine uptake and a parallel decrease in [³H]thymidine uptake.