Fig. 8. Efficient p52 induction by CD40 requires its TRAF2/3-binding site. (A) 293 cells were transiently transfected with vectors encoding the indicated CD40 chimeras. Cells were stimulated with anti-CD40 mAb for the last 16 h of a 48 h culture and lysates western blotted. Each of the CD40 chimeras was expressed at similar levels (data not shown). (B) 293 cells were co-transfected with vectors encoding CD40 and dominant-negative TRAFs or EV. Cells were stimulated for the last 16 h of a 48 h culture and lysates western blotted. Similar levels of CD40 were expressed in all transfections, and each of the TRAF dominant-negative proteins was expressed at comparable levels (data not shown).