Fig. 4. RNA analysis of the different pre-60S particles. (A) Primer extension and (B) northern hybridization analyses for 35S, 27S and 25S rRNA were performed on RNA extracted from whole cells (Total) and affinity-purified tagged (Nsa3, Nop7, Nug1, Rix1, Sda1, Arx1 and Kre35) and non-tagged (Negative) wild-type strain. RNA was loaded proportionally to a Coomassie stained protein SDS–PAGE from the same purifications. The oligonucleotide used for each panel is indicated on the left side of each gel (see Materials and methods). The annealing location of oligonucleotides used for primer extension and northern hybridization analysis are indicated (C).