Fig. 4. GFP–LimC and GFP–LimD redistribution during fluid-phase endocytosis. Cells expressing GFP–LimC (A) or GFP–LimD (B) were allowed to adhere to glass coverslips and the supernatant was replaced by phosphate buffer containing 1 mg/ml TRITC–dextran. Confocal sections were taken at the times indicated. Arrowheads in the 0 s panels indicate the site of formation of pinocytic vesicles and arrows in subsequent images indicate newly formed pinosomes. Bars, 10 µm.