Table II. Lateral pseudopod formation by AX2 wild-type and LimD^– cells crawling in buffer or in a spatial gradient of cAMP.

	Cell type	Number of cells	0–2 lateral pseudopods per 10 min (%)	3–5 lateral pseudopods per 10 min (%)	>5 lateral pseudopods per 10 min (%)	Average frequency of lateral pseudopods per cell per 10 min
Buffer	AX2	28	15	53	30	5.3
	LimD–	23	5	60	34	4.9
Gradient	AX2	22	81	18	–	1.45
	LimD–	25	40	60	–	2.7

Images were taken at 20× magnification every 30 s. In all cases cells were analyzed for 10 min. A χ² test was performed between AX2 and LimD^– cells on the combined data of the three categories of lateral pseudopods formed. The difference between AX2 and LimD^– in the cAMP gradient was found to be highly significant (1 × 10^–3).