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. 2005 Dec;25(23):10665–10673. doi: 10.1128/MCB.25.23.10665-10673.2005

FIG. 5.

FIG. 5.

Rfx1 represses the endogenous Rfx1 expression. Overexpression of HARfx1 ΔN represses the expression of endogenous Rfx1. A. Schematic drawing of Rfx1 indicating the location of PCR primers by numbers. Dim, dimerization domain. B. RT-PCR performed on RNA extracted from HEK293 cells transfected with HARfx1 ΔN (+) or with control empty pSG5 vector (−). The top panel shows the level of the endogenous Rfx1 RNA amplified with primers 2 and 3, while the middle panel shows the transfected HARfx1 ΔN amplified with primers 1 and 3. β-Actin was used as internal control (lower panel). C. Western blot on HEK293 cell extracts transfected with HARfx1 ΔN expression vector (middle panel). The level of the endogenous Rfx1 protein was determined using anti-Rfx1 antibodies (upper panel). β-Tubulin was used as a control (lower panel). IB, immunoblot.