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RFX1 gene expression is UV responsive. A. RT-PCR using total RNA from UV-irradiated MCF-7 cells. UCN-01 (1 μM) or caffeine (3 mM) was added 30 min before irradiation (20 J/m2). B. RT-PCR of MCF-7 stably expressing siRNA against Chk2, Chk1, or ATR irradiated with 20 J/m2 UV. C. A suggested model for the interaction of Rfx1 with the other components of the DNA replication checkpoint pathway.
