FIG. 11.

66S preribosomes are largely intact but lack Ytm1 in the ytm1-1 mutant. Wild-type YTM1 cells and mutant ytm1-1 cells expressing Nop7-TAP or Brx1-TAP were grown in YEPD medium at 25°C and shifted to 37°C for 5 h. (A) Silver staining or (B) Western immunoblot analysis was performed on proteins trichloroacetic acid precipitated from samples affinity purified from extracts from these strains.