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Ytm1-HA3 cosediments on sucrose gradients with 66S preribosomes. Whole-cell extracts were prepared from yeast strain JWY6770 (YTM1-HA3) and fractionated on 7 to 47% sucrose velocity gradients. Fractions containing 40S and 60S ribosomal subunits and 80S monosomes are labeled. Proteins were trichloroacetic acid precipitated from gradient fractions and subjected to Western immunoblot analysis to detect Ytm1-HA3.
