TABLE 2.
Nonribosomal proteins that copurify with Ytm1-TAP
| Gel ID no.a | Protein | ORF | Characteristic
|
Pre-rRNA processingc | Localizationb | Reference | |||
|---|---|---|---|---|---|---|---|---|---|
| Metazoan homolog | Essential | Sediments at 66S | Decreased 60S Subunits | ||||||
| 33 | Ytm1 | YOR272w | + | + | + | + | 27SA3↑ 25.5S↓ 7S↓ | No/Nu | This work (21) |
| 10 | Nip7 | YPL211w | + | + | + | + | NDd | No/Nu/C | 61 |
| 24 | Nop16 | YER002c | − | − | + | + | ND | No/Nu | 21 |
| 26 | Brx1 | YOL077c | + | + | ND | + | 27SA↑ 27SB↓ | No | 31 |
| 28 | Rlp7 | YNL002c | + | + | ND | + | 27SA3↑ 27SB↓ 7S↓ | No | 9,15 |
| 28 | Rpf2 | YKR081c | + | + | + | + | 27SB↑ | No | 39,60 |
| 29 | Cic1/Nsa3 | YHR052c | − | + | + | ND | 27S↓ 7S↓ | No | 13, 21,41 |
| 31 | Nsa1 | YGL111w | − | + | ND | + | ND | ND | 21 |
| 32, 33 | Has1 | YMR290c | + | + | ND | +/− | 35S↑ 27SB↑ | No | 10 |
| 34 | Ebp2 | YKL172w | + | + | ND | + | 35S↑ 27SA3↑ | No | 27,57 |
| 35, 36 | Nog1 | YPL093w | + | + | + | + | 27S↓ 7S↓ | No | 30 |
| 37 | Nop7 | YGR103w | + | + | + | + | 27S→25S slow | No | 1 |
| 37 | Noc3 | YLR002c | + | + | + | + | 35S↑ 27SB↑ | No/Nu | 37 |
| 38 | Noc2 | YOR206w | + | + | + | + | 35S↑ 27SB↑ | No/Nu | 37 |
| 38 | Nop2 | YGL111w | + | + | + | + | 35S↑ 27S↑ | No | 6,25 |
| 39 | Drs1 | YLL008w | − | + | ND | + | 27SB↑ | No | 48 |
| 40 | Erb1 | YMR049c | + | + | + | + | 27S↑ | No | 44 |
a
Protein band 30 was identified as Tef2 and protein band 36 also contained Ssal. These proteins are common contaminants of affinity-purified complexes (17,24).
b
No, nucleolar; Nu, nuclear; C, cytoplasmic.
c
↑ and ↓, increased and decreased amounts of indicated RNAs, respectively.
d
ND, not determined.