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Phosphorylation of caspase 9 (Casp-9) following hyperosmotic stress. U2OS (A and B) or HEK293 (C and D) cells were transfected with caspase 9 (C287A) or empty vector. At 24 h posttransfection, cells were serum starved for 24 h before treatment with 1 μM OA for 60 min or (A) 1 μM TPA, (B) 10% fetal calf serum (FCS), (C) 0.7 M NaCl, or (D) 0.5 M sorbitol. Cell lysates were analyzed by immunoblotting with phospho-Ser144 and caspase 9 antibodies as indicated.
