TABLE 1.
S. cerevisiae strains used in this studya
| Strain | Genotype | Source and/or reference |
|---|---|---|
| JKM179/120 | MATα/mata Δho/Δho Δhml::ADE1/HMLα Δhmr::ADE1/HMRa ade1-100/ade1-100 leu2-3,112/leu2-3,112 lys5 trp1::hisG/lys5 trp1::his ura3-52/ura3-52 ade3::GAL::HO/ADE3 | J. Haber |
| JKM120Ku | MATa Δho ade1-100 leu2-3,112 lys5 trp1::hisG′ ura3-52 yku70::LEU2 | J. Haber |
| JKM179Ku | MATα Δho Δhml::ADE1 Δhmr::ADE1 ade1-100 leu2-3,112 lys5 trp1::hisG′ ura3-52 ade3::GAL::HOyku70::LEU2 | J. Haber |
| YVG100 | JKM179/120, MEC1/mec1::KAN | This study |
| JKM YKU+/− | MATa/matα Δho/Δho Δhml::LEU2/Δhml::ADE1 HMRa/Δhmr::ADE1 ade1-100/ade1-100 leu2-3,112/leu2-3,112 lys5/lys5 trp1::hisG/trp1::hisG ura3-52/ura3-52 ADE3/ade3::GAL::HO YKU70/yku70Δ::URA3 | J. Haber |
| YVG101 | JKM YKU+/−, YKU70/yku70::LEU2 MEC1/mec1::KAN | This study |
| JKM SIR3 −/− | MATa/matα Δho/Δho HMLα/Δhml::LEU2 HMRa/Δhmr::ADE1 ade1-100/ade1-100 leu2-3,112/leu2-3,112 lys5/lys5 trp1::hisG/trp1::hisG ura3-52/ura3-52 ADE3/ade3::GAL::HO sir3Δ::URA3/sir3Δ::URA3 | J. Haber |
| YVG102 | JKM SIR3−/−, MEC1/mec1::KAN | This study |
| JKM YKU −/− | JKM179/120, yku70::LEU2/yku70::LEU2 | J. Haber |
| YVG103 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN | This study |
| YVG104 | JKM179/120, yku70::LEU2/yku70::LEU2 RAD53/rad53::NAT | This study |
| YVG105 | JKM179/120, yku70::LEU2/yku70::LEU2 RAD53/rad53-K227A::KAN | This study |
| YVG106 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN SIR3/sir3::TRP1 | This study |
| YVG107 | JKM179/120, SIR3/sir3::LEU2 MEC1/mec1::TRP1 | This study |
| YVG108 | JKM179/120, yku70::LEU2/yku70::LEU2 LCD1/lcd1::TRP1 | This study |
| YVG109 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::NAT | This study |
| YVG110 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::NAT RAD17/rad17::KAN | This study |
| YVG111 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN DDC1/ddc1::TRP1 | This study |
| YVG112 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN MEC3/mec3::TRP1 | This study |
| YVG113 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::NAT RAD24/rad24::KAN | This study |
| YVG114 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN MRC1/mrc1::TRP1 | This study |
| YVG115 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN CHK1/chk1::TRP1 | This study |
| YVG116 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN MAD2/mad2::TRP1 | This study |
| YVG117 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN EXO1/exo1::URA3 | This study |
| YVG118 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN RAD52/rad52::TRP1 | This study |
| YVG119 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN RAD9/rad9::TRP1 | This study |
| YVG120 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN RAD9/rad9::TRP1 SML1/sml1::NAT | This study |
| YVG121 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN MRE11/mre11::TRP1 | This study |
| YVG122 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN MRE11/mre11::TRP1 SML1/sml1::NAT | This study |
| YVG123 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN TEL1/tel1::TRP1 | This study |
| YVG124 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN TEL1/tel1::TRP1 SML1/sml1::NAT | This study |
| YVG125 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN tel1::URA3/tel1::TRP1 | This study |
| YVG126 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN DUN1/dun1::TRP1 | This study |
| YVG127 | JKM179/120, yku70::LEU2/yku70::LEU2 MEC1/mec1::KAN RNR3/rnr3::TRP1 | This study |
| YVG128 | JKM179/120, lif1::TRP1/lif1::TRP1 MEC1/mec1::KAN | This study |
| YVG130 | Spore est2::CLONAT arising from the dissection of JKM179/120, EST2/est2::CLONAT | This study |
| W303-1A | MATaade2-1 can1-100 his3-11,15 leu2-3,112 rad5-535 trp1-1 ura3-1 | R. Rothstein |
| W303-1a | MATα ade2-1 can1-100 his3-11,15 leu2-3,112 rad5-535 trp1-1 ura3-1 | R. Rothstein |
| YVG140 | W303-1A yku70::TRP1 | This study |
| YVG141 | W303-1A yku70::TRP1 mec1::KAN | This study |
| W303-1A-YFP-SML1 | MATaYFP-SML1 RAD5 ade2-1 can1-100 his3-11,15 leu2-3,112 rad5-535 trp1-1 ura3-1 | R. Rothstein |
| W303-1a-YFP-SML1 | MATα YFP-SML1 RAD5 ade2-1 can1-100 his3-11,15 leu2-3,112 rad5-535 trp1-1 ura3-1 | R. Rothstein |
| YVG142 | W303-1A-YFP-SML1 yku80::TRP1 | This study |
| YVG143 | W303-1A-YFP-SML1 yku80::TRP1 mec1::KAN | This study |
| YVG144 | W303-1A-YFP-SML1 mre11::URA3 | This study |
| YVG145 | W303-1A-YFP-SML1 yku80::TRP1 mre11::URA3 | This study |
| YVG146 | W303-1a-YFP-SML1 rad9::TRP1 | This study |
| YVG147 | YVG142 × YVG146, YFP-SML1/YFP-SML1 yku80::TRP1/YKU80 RAD9/rad9::TRP1 | This study |
| YVG148 | Spore YFP-SML1 yku80::TRP1 rad9::TRP1 arising from the dissection of YVG147 | This study |
| YVG149 | W303-1a-YFP-SML1 dun1::TRP1 | This study |
| YVG150 | YVG142 × YVG149; YFP-SML1/YFP-SML1 yku80::TRP1/YKU80 DUN1/dun1::TRP1 | This study |
| YVG151 | Spore YFP-SML1 yku80::TRP1 dun1::TRP1 arising from the dissection of YVG150 | This study |
| YVG152 | W303-1A-YFP-SML1 rad53-K227A::KAN | This study |
| YVG153 | W303-1A-YFP-SML1 yku80::TRP1 rad53-K227A::KAN | This study |
| YVG154 | W303-1A-YFP-SML1 tel1::URA3 | This study |
| YVG155 | W303-1A-YFP-SML1 yku80::TRP1 tel1::URA3 | This study |
| YVG156 | W303-1A-YFP-SML1 rif2::KAN | This study |
| YVG157 | W303-1A-YFP-SML1 yku80::TRP1 rif2::KAN | This study |
| YVG158 | W303-1A-YFP-SML1 tel1::URA3 rif2::KAN | This study |
| YVG159 | W303-1A-YFP-SML1 yku80::TRP1 tel1::URA3 rif2::KAN | This study |
| YVG160 | W303-1A-YFP-SML1 + pRS416-MEC1 | This study |
| YVG161 | W303-1A-YFP-SML1 yku80::TRP1 + pRS416-MEC1 | This study |
| YVG162 | W303-1A-YFP-SML1 yku80::TRP1 mec1::KAN + pRS416-MEC1 | This study |
| YVG163 | W303-1A-YFP-SML1 mec1::KAN + pRS416-MEC1 | This study |
| LPY917 | MATaade2-101 trp1Δ1 leu2Δ1 his3Δ-200 lys2-801 TELadh4::URA3 | L. Pillus |
| YVG164 | LPY 917 yku70::TRP1 | This study |
| YVG165 | LPY 917 yku80::TRP1 | This study |
| YVG166 | LPY 917 yku80::TRP1 + pRS413-YKU80 | This study |
| YVG167 | W303-1a-YFP-SML1 yku80::TRP1 | This study |
| YVG168 | LPY 917 yku80::TRP1 + pRS413-yku80PF437,438AA | This study |
| YVG169 | LPY 917 yku70::TRP1 mec1::KAN | This study |
| YVG170 | LPY917 yku80::TRP1 mec1::KAN | This study |
| YVG171 | LPY 917 sml1::NAT | This study |
| YVG172 | LPY 917 sml1::NAT mec1::KAN | This study |
| W303-1A | MATaade2-1 can1-100 his3-11,15 leu2-3,112 rad5-535 trp1-1 ura3-1 | R. Rothstein |
| W3483-10A | MATaMRE11-YFP | R. Rothstein, 27 |
| YVG173 | W3483-10A × YVG167, MRE11-YFP/MRE11 SML1/YFP-SML1 YKU80/yku80::TRP1 | This study |
| YVG174 | Spore MRE11-YFP yku80::TRP1 arising from the dissection of YVG173 | This study |
| YVG175 | Spore MRE11-YFP arising from the dissection of YVG173 | This study |
| W1379-3C | MATabar1Δ::LEU2 | R. Rothstein, 57 |
| W2057-11A | MATabar1Δ::LEU2 GAL-SML1 | R. Rothstein, 57 |
a
Most yeast strains used in this study have an isogenic JKM, W303, LPY917, or YPH250 background. Relevant genotypes are shown. Where strains are the products of a genetic cross, the numbers of the parent strains are also indicated.