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. 2005 Dec;25(23):10479–10491. doi: 10.1128/MCB.25.23.10479-10491.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 4. — Expression of cell type-specific markers in trophoblast tissue. (A) E9.5 placentas analyzed by ³⁵S in situ hybridization for Tpbp and Pl 1 in Hif1α^+/+ Hif2α^+/+ (a and e), Hif1α^+/− Hif2α^+/⁻ (b and f), Hif1α^−/− Hif2α^+/⁻ (c and g), and Hif1α⁻^/⁻ Hif2α^−/− (d and h) embryos. Original magnification, ×50. (B) E9.5 placentas analyzed by ³⁵S in situ hybridization for expression of TFEB in Arnt^+/+ (a and e), Arnt⁻^/⁻ (b and f) Hif1α^+/+ Hif2α^+/+ (c and g), and Hif1α⁻^/⁻ Hif2α⁻^/⁻ (d and h) embryos. Antisense probes are shown in a, b, c, and d, and sense probes are shown in e, f, g, and h. Original magnification, ×50. Arrowheads indicate cells expressing TFEB.