FIG. 5.
Generation and differentiation of trophoblast stem (TS) cells. (A) Wild-type undifferentiated TS cells on MEFs or in MEF-conditioned media (lanes 1 and 2), differentiated TS cells (lane 3), MEFs alone, and R1 ES cells were analyzed by RT-PCR for ERRβ, FGFR2, Tpbp, Mash2, Pl 1, Oct-4, and HPRT. (B) Arnt+/+ and Arnt−/− TS cells were differentiated and examined by Northern blot assay for expression of Tpbp and Pl 1 under 20% O2 and 3% O2. U, undifferentiated (undiff) cells; D, differentiated (diff) cells. (C) Hif1α+/+ Hif2α+/+ and Hif1α−/− Hif2α−/− TS cells were differentiated and examined by Northern blot for Tpbp and Pl 1 expression.