FIG. 2.

The KQQKK sequence at the Ftz-F1 box of SF-1 is acetylated by p300 in vivo. (A) Potential acetylation sites of SF-1. The amino acid sequences of two potential acetylation sites of SF-1 are shown, and the conserved Lys (K) residues are marked. In mutated SF-1 (mt1, mt2, and dmt), the K residues were replaced with Arg (R). F, Ftz-F1 box; P, proline-rich domain; and LBD, ligand-binding domain. (B) Mutation of KQQKK sequence abolishes p300-enhanced acetylation of SF-1. The incorporation of [³H]acetate into wild-type (WT) and mutated SF-1 following expression and immunoprecipitation (IP) of SF-1-HA are shown; the amounts of immunoprecipitated SF-1 and p300 expression were determined by SF-1 and c-Myc immunoblotting (IB), respectively.