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. 2005 Dec;25(23):10442–10453. doi: 10.1128/MCB.25.23.10442-10453.2005

FIG. 6.

FIG. 6.

p300 recruits SF-1 to p300-RNA Pol II loci. (A) SF-1 partially colocalizes with p300 in the basal condition. Y1 cells grown on coverslips were fixed and labeled with antibodies against SF-1 (red) and p300 (green). Immunofluorescence staining was analyzed by confocal laser scanning microscopy. The area marked by a rectangle is enlarged in panel B. The arrows indicate yellow colors as a result of colocalization of SF-1 and p300. (C) p300 foci contain RNA Pol II. Expression plasmid for c-Myc-tagged p300 was transfected into Y1 cells. Fixed cells were labeled with antibodies against RNA Pol II (red) and c-Myc (green). An optical section of immunofluorescence staining is shown. (D) The HAT activity of p300 is not required to recruit SF-1 to p300-foci. Y1 cells were transfected with expression plasmid for c-Myc-tagged p300 WT (top), p300 DY (middle), or FLAG-tagged GCN5 (F-GCN5) (bottom). Fixed cells were labeled with antibodies against SF-1 (red), c-Myc (green), or FLAG (green). Optical sections of immunofluorescence staining are shown.