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cAMP increases SF-1/p300 association in Y1 cells. The expression plasmids for wild-type (WT) 3xFLAG-SF-1 (3xF-SF-1) or mutated mt1 were transfected into Y1 cells. After 24 h, cells were treated with (+) or without (−) 1 mM 8-Br-cAMP for 6 h. Results of immunoblotting (IB) of SF-1 and p300 recovered from immunoprecipitates (IP) of cell lysates using anti-p300 antibody are shown. Immunoblotting of SF-1, p300, and Pol II in 10% of input cell lysate (Input) are shown at the bottom.
