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. 2005 Dec;25(23):10580–10590. doi: 10.1128/MCB.25.23.10580-10590.2005

FIG. 2.

FIG. 2.

Identification of mitotic phosphorylation sites in Wee1A. (A) Coomassie-stained gels of recombinant Wee1A incubated with buffer or extracts. (B) Summary of the peptides identified by MALDI-TOF MS. Coverage ranged from 44 to 60%. Peptides containing 5 of the 11 SP/TP sites were detected. WT, wild type. (C) MALDI-TOF MS spectrum of trypsin-digested interphase Wee1A. Three nonphosphorylated peptide peaks are indicated. Their observed m/z ratios are 875.4405 (identified as GSPVSSWR; theoretical m/z = 875.4375), 1,458.7101 (identified as TNNCPFPITPQR; theoretical m/z = 1,458.7164), and 2,809.3623 (identified as FVAGTGAELDDPSLVNINPFTPESYR; theoretical m/z = 2,809.3685). (D) MALDI-TOF MS spectrum of trypsin-digested M-phase Wee1A. Three phosphorylated peptide peaks are indicated. Their observed m/z ratios are 1,538.6667 [identified as TNNCPFPITPQR(-PO4); theoretical m/z = 1,538.6827], 1,937.8879 [identified as TNNCPFPITPQRNER(-PO4); theoretical m/z = 1,937.8693], and 2,889.3784 [identified as FVAGTGAELDDPSLVNINPFTPESYR(-PO4); theoretical m/z = 2,889.3348].