TABLE 2.
Plasmid construct list
| Plasmid | Description | Source or reference |
|---|---|---|
| pMK125 | pRS414-GCN5 | 52 |
| pMK284 | Integration construct for introducing point mutations to GCN5 | This study |
| pMK284E173H | Integration construct for introducing E173H to GCN5 | This study |
| pMK284F221A | Integration construct for introducing F221A to GCN5 | This study |
| pMK334 | Integration construct for introducing HIS3-lacZ reporter to URA3 locus | This study |
| pMK449 | pBluescriptKS-SNF1 | This study |
| pMK453 | snf1Δ::LEU2 disruptor | This study |
| pKD97 | pRS316-HA-reg1Δ6 | 23 |
| pYL31 | pRS316-HA-REG1(1-740) | This study |
| pYL35 | Integration construct for introducing REG1(1-740) mutation | This study |
| pYL41 | YEplacI12-SNF1 | This study |
| pYL42 | pYEX-4T-GST-Snf1 | 35 |
| pYL44 | pYEX-4T-GST | A. Acharya, unpublished |
| pYL45 | snf1Δ::TRP1 disruptor | This study |
| pYL54 | pYEX-4T-Gcn5-TAP | This study |
| pYL67 | 8 × Myc::TRP1 for tagging proteins with 8 Myc repeats | This study |
| pQQ18 | pRS315-HTA1-HTB1 HHT2-HHF2 | 2 |
| pJH33 | pRS316-HTA1-HTB1 HHT2-HHF2 | 2 |
| pMK439S10A | pQQ18 with an H3 S10A mutation | This study |
| pMK439S10E | pQQ18 with an H3 S10E mutation | This study |
| pMK439S10A/S28A/S31A | pQQ18 with an H3 S10A/S28A/S31A mutation | This study |
| pMK439S10E/S28D/S31D | pQQ18 with an H3 S10E/S28D/S31D mutation | This study |
| pMK515 | pET21-6xHis-Gcn5 protein | This study |
| pMK547Gcn5 | 3xHA-Gcn5 overexpression | This study |