Wild-type percentages of thymic and peripheral lymphocyte populations in Cbp-deficient mice. Total thymocytes, splenocytes, and lymph node cells were stained with the indicated antibodies and analyzed by flow cytometry. Numbers indicate percentages of gated cells. The dotted line in each histogram represents double-positive cells, the gray area represents CD8 single-positive cells, and the black area represents CD4 single-positive (SP) cells. Abbreviations: Thy, thymus; DN, gated on CD4− and CD8− (double-negative) cells; Spl, spleen; LN, lymph nodes, CD4+, gated on CD4+ cells; +/+, Cbp+/+; −/−, Cbp−/−.