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. 2005 Dec;25(23):10205–10219. doi: 10.1128/MCB.25.23.10205-10219.2005

FIG. 8.

FIG. 8.

The presence of C/EBPα partially relieves cyclin D3 inhibition on AML1. A. K562 cells were transfected with the indicated expression constructs as described for Fig. 5 with or without 2 μg of pCMV5-C/EBPα in the presence or absence of 4 μg pRcCMV-cyclin D3-HA. The luciferase results were normalized to the expression of Renilla luciferase. The averages and standard deviations were generated from results from duplicates of two independent experiments. B. One hundred percent induction was set for AML1 or for AML1 and C/EBPα in the absence of cyclin D3. The averages and standard deviations were generated from results of duplicates of two independent experiments. C. Cyclin D3 and C/EBPα do not compete with each other in their interaction with AML1. 293T cells were transfected with 5 μg of pRSV-cyclin D3 and 5 μg of pcDNA6-HA-AML1 with the addition of 5 or 15 μg of pMSV-C/EBPα and equilibrated to 25 μg with the empty vector. HA-tagged AML1 protein was immunoprecipitated (IP) with anti-HA antibody (αHA), and Western blotting (WB) was performed with the indicated antibodies (top panels). Straight Western blotting of 10 μg of each sample is shown (bottom panels).