FIG. 1.

2-D DIGE overlay image of the Y. pestis soluble proteome showing differential protein expression between two in vitro growth conditions containing different temperatures and calcium levels. Colored spots represent proteins that are more abundant at 26°C with 4 mM calcium (red), at 37°C with 0 mM calcium (blue), or in the pooled standard (green). White spots represent proteins with unaltered expression across the gel. Arrows with numbers show the locations of differentially expressed proteins identified by mass spectrometry (listed in Table 1).