TABLE 1.
Differentially expressed Y. pestis proteins related to virulence
| Spot no. | Protein | Accession no. | Mola | pIa | Methodb | Fold change
|
|||
|---|---|---|---|---|---|---|---|---|---|
| 26°C, 4 mM Ca2+ vs 37°C, 0 mM Ca2+ | 26°C vs 37°C at 4 mM Ca2+ | 4 vs 0 mM Ca2+ | 26°C vs 37°C | ||||||
| 1 | Catalase-peroxidase (α-KatY) | NP_668204 | 81.5 | 6.9 | MALDI | 2.5 | 5.1 | −1.7 | 3.0 |
| 2 | Catalase-peroxidase (α-KatY) | NP_668204 | 81.5 | 6.9 | MALDI | 3.5 | 4.5 | −1.3 | 3.3 |
| 3 | Catalase-peroxidase (α-KatY) | NP_668204 | 81.5 | 6.9 | MALDI | 4.1 | 6.1 | −1.4 | 4.0 |
| 4 | Clp ATPase (ClpB) | NP_668245 | 95.8 | 5.6 | MALDI | 2.9 | 2.6 | 1.2 | 3.1 |
| 5 | Clp ATPase (CIpB) | NP_668245 | 95.8 | 5.6 | MALDI | 2.3 | 1.7 | 1.0 | 1.6 |
| 6 | 60-Da chaperonin (Cpn60) | NP_667946 | 57.4 | 4.9 | MALDI | 1.7 | 1.3 | 1.3 | 1.3 |
| 7 | Murine toxin (Ymt) | NP_857852 | 67.5 | 5.6 | LC/MS/MS | 2.3 | 1.3 | 1.5 | 1.9 |
| 8 | Catalase-peroxidase (β-KatY) | NP_668204 | 53.6 | 5.8 | LC/MS/MS | 3.3 | 8.0 | −1.1 | 7.0 |
| 9 | Catalase-peroxidase (β-KatY) | NP_668204 | 53.6 | 5.9 | MALDI | 7.4 | 3.5 | −1.3 | 3.2 |
| 10 | Aspartate aminotransferase (AspC) | NP_670061 | 43.7 | 5.1 | MALDI | 3.3 | 2.5 | 1.3 | 2.5 |
| 11a | High-temperature protein G (HtpG) | NP_668394 | 70.8 | 5.0 | MALDI | 3.1 | 2.4 | 1.2 | 2.7 |
| 11b | Phosphoglycerate kinase (Pgk) | NP_670607 | 41.2 | 5.3 | MALDI | 3.1 | 2.4 | 1.2 | 2.7 |
| 12 | Maltose binding protein periplasmic protein precursor (MalE) | NP_667372 | 43.8 | 6.2 | De novo sequencing | −1.3 | −1.4 | 1.3 | −1.6 |
| 13 | Fructose-bisphosphate aldolase class II (Fba) | NP_670606 | 39.3 | 5.7 | LC/MS/MS | −1.5 | −1.3 | 1.2 | −1.6 |
| 14 | 6-Phosphogluconate dehydrogenase (Gnd) | NP_669932 | 53.4 | 5.3 | De novo sequencing | 3.5 | 1.9 | 1.4 | 2.3 |
| 15 | α-Enolase (Eno) | NP_668150 | 45.5 | 5.2 | LC/MS/MS | 4.9 | 4.4 | 1.6 | 4.2 |
| 16 | d-3-Phosphoglycerate (SerA) | NP_670600 | 44.5 | 5.8 | LC/MS/MS | 5.9 | 4.5 | 1.1 | 4.7 |
| 17 | 60-kDa chaperonin (Cpn60) | NP_667946 | 57.4 | 4.9 | LC/MS/MS | 4.1 | 3.7 | −1.1 | 3.7 |
| 18 | Plasminogen activator (Pla) | NP_857784 | 34.6 | 6.1 | MALDI | 2.8 | 2.4 | 1.1 | 2.4 |
| 19 | F1 capsule antigen (Caf1) | NP_857692 | 17.7 | 4.8 | De novo sequencing | 2.4 | 2.2 | 1.0 | 2.1 |
| 20 | Succinyl-CoA synthetase, alpha chain (SucD) | NP_992413 | 30.0 | 6.1 | De novo sequencing | 4.2 | 5.1 | −1.0 | 4.0 |
| 21 | 60-kDa chaperonin (Cpn60) | NP_667946 | 57.4 | 4.9 | LC/MS/MS | 4.2 | 4.3 | 1.0 | 6.6 |
| 22 | 60-kDa chaperonin (Cpn60) | NP_667946 | 57.4 | 4.9 | LC/MS/MS | 7.2 | 3.8 | 1.2 | 3.1 |
| 23 | 60-kDa chaperonin (Cpn60) | NP_667946 | 57.4 | 4.9 | MALDI | 8.2 | 5.3 | 1.2 | 4.6 |
| 24 | 60-kDa chaperonin (Cpn60) | NP_667946 | 57.4 | 4.9 | LC/MS/MS | 5.3 | 3.8 | 1.1 | 6.6 |
| 25 | Outer membrane porin A (OmpA) | NP_992692 | 38.0 | 6.8 | LC/MS/MS | 12.3 | 8.0 | 1.3 | 6.2 |
| 26 | Catalase-peroxidase (γ-KatY) | NP_668204 | 36.0 | 5.6 | MALDI | 8.9 | 6.0 | 1.3 | 6.0 |
| 27 | 60-kDa chaperonin (Cpn60) | NP_667946 | 57.4 | 4.9 | MALDI | 3.2 | 2.0 | 1.8 | 1.5 |
| 28 | Probable N-acetylmuramoyl-l-alanine amidase (AmpD3) | NP_993160 | 28.7 | 5.5 | MALDI | 2.7 | 2.3 | 1.2 | 1.8 |
| 29 | Murine toxin (Ymt) | NP_857852 | 67.7 | 5.6 | LC/MS/MS | 3.3 | 1.8 | 1.7 | 1.4 |
| 30 | 50S ribosomal protein L3 (RplC) | NP_991606 | 22.3 | 9.9 | LC/MS/MS | 8.3 | 4.8 | 1.7 | 4.6 |
| 31 | 50S ribosomal protein L3 (RplC) | NP_991606 | 22.3 | 9.9 | LC/MS/MS | 10.6 | 4.2 | 2.0 | 7.5 |
| 32 | 50S ribosomal protein L3 (RplC) | NP_991606 | 22.3 | 9.9 | LC/MS/MS | 2.5 | 1.7 | 1.0 | 1.6 |
| 33 | Elongation factor Ts (EF1B) | NP_670435 | 30.8 | 5.2 | LC/MS/MS | 3.3 | 2.6 | 1.0 | 2.5 |
| 34a | 50S ribosomal protein L3 (RplC) | NP_991606 | 22.3 | 9.9 | LC/MS/MS | 1.8 | 1.4 | 1.0 | 1.8 |
| 34b | Elongation factor Ts (EF1B) | NP_670435 | 30.8 | 5.2 | LC/MS/MS | 1.8 | 1.4 | 1.0 | 1.8 |
| 35 | 60-kDa chaperonin (Cpn60) | NP_667946 | 57.4 | 4.9 | LC/MS/MS | −1.2 | −1.1 | −1.1 | −1.1 |
| 36 | 30S ribosomal protein SI (RpsA) | NP_670081 | 61.4 | 5.0 | LC/MS/MS | 2.0 | 1.6 | 1.1 | 1.9 |
| 37 | 60-kDa chaperonin (Cpn60) | NP_667946 | 57.4 | 4.9 | LC/MS/MS | 1.5 | 1.1 | 1.2 | 1.5 |
| 38 | Catalase-peroxidase (δ-KatY) | NP_668204 | 34.0 | 5.6 | MALDI | 5.5 | 3.5 | 1.4 | 4.6 |
| 39 | Trp repressor binding protein (WrbA) | NP_669755 | 20.8 | 8.0 | LC/MS/MS | 1.4 | 1.4 | −1.3 | 1.7 |
| 40 | Glyceraldehyde 3-phosphate dehydrogenase A (GapA) | NP_669476 | 36.0 | 6.2 | LC/MS/MS | 2.0 | 1.5 | 1.2 | 1.8 |
| 41 | d-Ribose-5-phosphate 3-epimerase (Rpe) | NP_671231 | 25.0 | 5.4 | LC/MS/MS | 1.2 | 1.3 | 1.1 | 1.1 |
| 42 | 50S ribosomal protein L6 (RplF) | NP_991621 | 19.0 | 9.7 | LC/MS/MS | 4.1 | 3.1 | 1.2 | 4.7 |
| 43 | 60-kDa chaperonin (Cpn60) | NP_667946 | 57.4 | 4.9 | De novo sequencing | 3.5 | 1.5 | 1.6 | 2.5 |
a
pI values and molecular weights in italics represent reported values for the full-length protein.
b
MALDI, matrix-assisted laser desorption ionization; LC/MS/MS, liquid chromatography-tandem mass spectrometry.