TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| B. subtilis strains | ||
| 168 | trpC2 | 4 |
| ML6 | trpC2 sigBΔHindIII-EcoRV::Cmr | 32 |
| 168-PR | trpC2 phoRΔBalI::Tcr | 61 |
| 168-MLPR | trpC2 sigBΔHindIII-EcoRV::CmrphoRΔBalI::Tcr | 61 |
| BFS1243 | trpC2 yurI::Emr | MICADOa |
| YFKNd | trpC2 yfkN::Emr | MICADO |
| YBCOdd | trpC2 ybcO::Emr | MICADO |
| BFS436 | trpC2 yjdB::Emr | MICADO |
| BFA1234-PR | trpC2 phoRΔBalI::TcryurI::Emr | This study |
| YFKNd-PR | trpC2 phoRΔBalI::TcryfkN::Emr | This study |
| YBCOdd-PR | trpC2 phoRΔBalI::TcrybcO::Emr | This study |
| BFA1243-ML | trpC2 sigBΔHindIII-EcoRV::CmryurI::Emr | This study |
| YFKNd-ML | trpC2 sigBΔHindIII-EcoRV::CmryfkN::Emr | This study |
| YBCOdd-ML | trpC2 sigBΔHindIII-EcoRV::CmrybcO::Emr | This study |
| YBCOdd-MLPR | trpC2 sigBΔHindIII-EcoRV::CmrphoRΔBalI::TcrybcO::Emr | This study |
| E. coli strain XL1-Blue | supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 lac [F′ proAB+lacIqlacZΔM15 Tn10 (Tcr)] | Stratagene Europe |
| Plasmids | ||
| pMUTIN4 | Apr EmrspoVG-lacZ Pspac (8.61 kb) | 79 |
| pBluescript II KS(+) | Cloning vector, Apr | Stratagene Europe |
| pphoA | pBluescript II KS(+) containing a 978-bp insert of phoA Apr | This study |
| pyheK | pBluescript II KS(+) containing a 485-bp insert of yheK Apr | This study |
a
MICADO, Microbial Advanced Database Organization.