TABLE 1.
Yeast two-hybrid interaction analysisa
| Bait protein | Domain | Prey protein and domain
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| RedC, HisKA | RedD, REC1 | RedD, REC2 | RedE, HisKA | RedF, REC | FrzZ, REC1 | FrzZ, REC2 | RNM | ||
| RedC | HisKA | ++ | ± | + | − | ± | − | − | − |
| RedD | REC1 | − | − | − | ++ | − | − | − | − |
| RedD | REC2 | − | ± | ± | ± | − | − | − | − |
| RedE | HisKA | − | + | − | − | − | − | − | − |
| RedF | REC | − | − | − | ++ | − | − | − | − |
| FrzZ | REC1 | − | − | − | − | − | − | − | − |
| FrzZ | REC2 | − | − | − | − | − | − | − | − |
| QN | − | − | − | − | − | − | − | ++ | |
Plasmids encoding B42 activation domain (prey, pJG4-5) (6) fusions to the histidine kinase (HisKA) domains of RedC (pLSE16) and RedE (pLSE19) and the receiver (REC) domains of RedD (REC1, pLSE17; REC2, pLSE18) and RedF (pLSE20) were paired with plasmids encoding the respective LexA binding domain (bait, pEG202) (6) fusions (pLSE06, pLSE07, pLSE08, pLSE09, or pLSE10) in the Saccharomyces cerevisiae EGY48/pSH18-34 lacZ reporter strain (4, 6) and tested for relative beta-galactosidase activity in the presence of galactose and 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside after 72 h of growth. Strong positive interactions (++) were defined by RNW (pDEW020) and QN (pDEW002) (2). +, positive interaction; ±, insignificant interaction; −, no interaction.