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Expression and purification of PlmS2 from recombinant Streptomyces coelicolor. A. SDS-PAGE of an intracellular extract of S.coelicolor carrying pMSG3 (lane 1) and a control plasmid, pSE34 (lane 2). B. SDS-PAGE of Ni-NTA column-purified polyhistidine-tagged PlmS2. C. Immunoblot of N-terminal polyhistidine-tagged PlmS2 with anti-polyhistidine antibodies.
