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. 2005 Dec;187(23):7970–7976. doi: 10.1128/JB.187.23.7970-7976.2005

TABLE 1.

Strains and plasmids used in this study

Strain or plasmid Descriptiona Reference or source
Strains
    Streptomyces sp. strain HK803 Wild type Riken, Japan
        NP1 mutant plmS2 replaced by Amr 17
        NP2 mutant plmS2-deleted mutant; Ams Unpublished
    S. coelicolor M115 15
    E. coli TG2 supE hsd5 thi (lac-proAB) (srl-recA) 306::Tn10(tetr) F[traD36 proAB+laclq lac ZM15] 22
    E. coli ET12567 Methylation-deficient E. coli host John Innes Center
    E. coli ET12567/pUZ8002 Nonmethylating E. coli harboring nontransmissible pUZ8002 (plasmid capable of mobilizing oriT-containing vectors) John Innes Center
Plasmids
    pET15b E. coli expression vector, Apr Novagen
    pMSG pET15b with 1.2-kbp NdeI-BamHI plmS2 ORF This work
    pGF200 pSET152 with 2.2-kbp EcoRI S. collinus ccr fragment from pHL18 13
    pMSG1 pGF200 with 0.7-kbp NdeI-BglI fragment from ccr replaced by 1.2-kbp NdeI-BamHI plmS2 This work
    pSE34 pWHM3 with ermE* promoter Pfizer Inc.
    pMSG3 pSE34 with 1.6-kbp XbaI-HindIII fragment from pMSG This work
a

Amr, apramycin resistance; Ams, apramycin sensitivity; Apr, ampicillin resistance.