TABLE 2.
Primers used for this study
| Primer | Sequencea (5′-3′) |
|---|---|
| Orf1-H3-R | gctctatAagctTaatgtatg |
| CRler-260 | gaaaaatggAtCcgttacgt |
| CRler-200 | cctggaTCCttgatctga |
| CRler-160 | caatacggAtcCggcgagccg |
| CRler-120 | attaatggaTCCacaata |
| CRler-80 | actagctGGatcCttataat |
| CRler-40 | tttttaattggGatCCtttt |
| CR-ORF10-HIII-A | cccacaggaaGcttcattac |
| CR-ORF10-HIII-B | ctgacataaGcTtcaacaaataac |
| CR-ORF11-H3 | tatacagaAgctTaccattgtaa |
| CR-ORF10-BHI | tgcacccacggGatcccacg |
| CR-ORF11-BHI | atttcctctgtgGatcCggggg |
| CR-RORF3-BH | aaacaatcagaagGatCCcaaaagttagtg |
| Cler-RBS-F | catgtaaggatCCgcttgttaa |
| ClerOrf1-R | gttcagttaaGCTtatcattta |
| CROrf11Xho | cagatttCtcgaGccgttaattat |
| EpCiorf11R-H3 | tactaagaAagcttcgtctaactctcc |
| ompAPE | tttgcgcctcgttatcatccaa |
| hnsH1P1 | cacccaatataagtttgagattactacaatgagcgaagctgtaggctggagctgcttcg |
| hnsH2P2 | gattttaagcaagtgcaatctacaaaagattattgcttcatatgaatatcctcctt |
| hnsM | tgcgagctcatcggtgtaa |
| hnsG | ttgctggcaaaaacccctccg |
a
Capital letters indicate changes in the oligonucleotide sequence with respect to the wild-type sequence, designed to introduce restriction enzyme sites.