TABLE 3.
Expression of rsmA71-lacZ and rsmA153-lacZ fusions in E. coli in the presence of expR71 or expR153
| Bacterial constructa | Relevant characteristicb | β-Galactosidase activity (Miller units)c |
|---|---|---|
| MC4100(pCL1920, pAKC1100) | Vector + rsmA71-lacZ | 620 ± 13 |
| MC4100(pAKC936, pAKC1100) | expR71 + rsmA71-lacZ | 1,905 ± 24 |
| MC4100(pAKC937, pAKC1100) | expR153 + rsmA71-lacZ | 7,465 ± 27 |
| MC4100(pCL1920, pAKC1106) | Vector + rsmA153-lacZ | 715 ± 16 |
| MC4100(pAKC936, pAKC1106) | expR71 + rsmA153-lacZ | 2,222 ± 48 |
| MC4100(pAKC937, pAKC1106) | expR153 + rsmA153-lacZ | 7,761 ± 32 |
a
Bacteria were grown at 28°C in LB agar supplemented with spectinomycin and tetracycline to a Klett value of ca. 250, and the whole cultures were used for the assay.
b
The relevant genes carried by bacteria are given.
c
Values are means±standard deviations of three repetitions.