TABLE 2.
Primers used to detect ETT2 genes in E. coli strains
| Primer designation | Sequence | Target genes in O157:H7 | Region designation | Resulting product length (kb) |
|---|---|---|---|---|
| 3715F | 5′ GCCGGGTTTGTATCGTGCCTTTTC 3′ | eprK-epaS | A | 5.4 |
| 3722R | 5′ TGCCGTTATGCTGGGTGGTGAG 3′ | |||
| 3721sF | 5′ CCGATTTCCTTTGCATATTTCC 3′ | epaR-epaO | B | 2.7 |
| 3727R | 5′ ATGCCAATGTGCGCGAAAATGAAT 3′ | |||
| 3726F | 5′ GCGCGCCATTTACACGTATCTCTA 3′ | eivJ-eivI | C | 2.7 |
| 3730R | 5′ TGGCGACAACCGTGGCAGAGTATT 3′ | |||
| 3730F | 5′ CCGCGTCATTGAGTCGATAAAAAG 3′ | eivC-eivF | D | 1.8a |
| 3735R | 5′ GGCCGCTTAGTCTCATTCCTTCC 3′ |
a
This is the region containing the deletion, as PCR with the same primers on a template of the O157:H7 genome is expected to result in a product of about 6.8 kb.