TABLE 2.

Summary of ToxCast bioassays associated with mechanisms of DART. The detailed assay information is provided in Supplementary Table S1.

Mode of action (NTP ICE)	AEID	ToxCast assay name	Intended target gene	No. of chemicals	Active chemicals (%)
Angiogenic process	174	BSK_4H_MCP1	CCL2	1705	407 (23.87%)
	196	BSK_BE3C_PAI1	SERPINE1	1705	306 (17.95%)
	250	BSK_hDFCGF_PAI1	SERPINE1	1705	430 (25.22%)
	258	BSK_hDFCGF_VCAM1	VCAM1	1705	451 (26.45%)
Aryl hydrocarbon receptor modulation	63	ATG_Ahr_CIS	AHR	4,039	692 (17.13%)
Estrogen metabolic process	907	CEETOX_H295R_ESTRADIOL	ESR1	576	74 (12.85%)
	909	CEETOX_H295R_ESTRONE	ESR1	576	90 (15.63%)
Estrogen receptor modulation	75	ATG_ERE_CIS	ESR1	4,039	848 (21%)
	786	TOX21_ERa_BLA_Antagonist_ratio	ESR1	8,305	1,053 (12.68%)
	2,119	TOX21_ERb_BLA_Antagonist_ratio	ESR2	7,871	1,459 (18.54%)
	2,211	TOX21_ERa_LUC_VM7_Agonist_10 nM_ICI182780	ESR1	7,871	159 (2.02%)
Estrogen-related receptor modulation	2070	TOX21_PGC_ERR_Antagonist	ESRRA	7,871	979 (12.44%)
Extracellular matrix	166	BSK_3C_uPAR	PLAUR	1705	443 (25.98%)
	232	BSK_CASM3C_uPAR	PLAUR	1705	266 (15.6%)
	256	BSK_hDFCGF_TIMP1	TIMP1	1705	300 (17.6%)
	274	BSK_KF3CT_TIMP2	TIMP2	1705	339 (19.88%)
Other developmental signaling transcription factors	66	ATG_BRE_CIS	SMAD1	4,039	365 (9.04%)
Oxidative stress	82	ATG_HIF1a_CIS	HIF1A	4,039	400 (9.9%)
p53 signaling pathway	1,317	TOX21_p53_BLA_p2_ratio	TP53	8,305	828 (9.97%)
Progesterone metabolic process	895	CEETOX_H295R_OHPROG	PGR	576	243 (42.19%)
	913	CEETOX_H295R_PROG	PGR	576	224 (38.89%)
Progesterone receptor modulation	2,127	TOX21_PR_BLA_Antagonist_ratio	PGR	7,871	2008 (25.51%)
Retinoic acid receptor modulation	71	ATG_DR5_RAR_CIS	RARA	4,039	309 (7.65%)