TABLE 1.

List of plasmids and strains

Strain or plasmid	Relevant characteristic or genotype	Reference
Plasmids
pBR322	E. coli plasmid cloning vector	2
pMUTIN2	B. subtilis integration plasmid for gene inactivation	33
pDG1728	B. subtilis plasmid for ectopic integration of lacZ transcriptional fusions at amyE	10
pEpUCΔ1	E. coli-B. subtilis shuttle vector to generate in-frame mutations	31
pTH1	Internal 348-bp fragment of thiL (ydiA) cloned into pMUTIN2	This work
pTH12	712-bp thiC promoter fragment cloned into pDG1728	This work
pTH30	In-frame ΔthiL (ydiA) deletion (G79-G202) cloned into pEpUCΔ1	This work
pTH31	Internal 353-bp yuaJ fragment cloned into pMUTIN2	This work
Strains
B. subtilis
PY79	SPβc (cured) prototroph	BGSC (1A747)
TH12	SPβc ΔthiL::cat4	This work
TH21	SPβc ΩamyE::thiC-lacZ	This work
TH22	SPβc ΔthiL::cat4 ΩamyE::thiC-lacZ	This work
TH48	ΔthiL::cat4 yuaJ (the first lesion in Tx26) ykoD (the second lesion in Tx26)	This work
TH76	ΩyuaJ::pMUTIN2	This work
TH83	ΔthiL yuaJ ykoD	This work
TH95	ΔthiL thiN (tx1) yuaJ ykoD ΩyloA::Tn917	This work
TH105	ΔthiL::cat4	This work
TH106	ΔthiL::cat4Ω thiL::pMUTIN2	This work
TH112	ΔthiL::cat4 ΩamyE::thiC-lacZ ykoD ΩyufR::Tn917	This work
TH128	ΔispA::neo	This work
TH137	ΩyuaJ::pMUTIN2 ΔykoD::cat4	This work
TH138	ΔykoD::cat4	This work
1A603	(SPβc2) trpC2 ΩthiC84::Tn917	34
1A631	(SPβc2) trpC2 ΩmotA::Tn917	34
1A633	(SPβc2) trpC2 ΩyloA::Tn917	34
1A642	(SPβc2) trpC2 ΩyufR::Tn917	34
Tx1	ΔthiL::cat4 ΩamyE::thiC-lacZ thiN (tx1)	This work
Tx26	ΔthiL::cat4 ΩamyE::thiC-lacZ yuaJ ykoD	This work
RL2066	ΔspoVM::spec	5
E. coli
PT-R1	thiO-35	E. coli Genetic Stock Center; 13
K12	Wild type	E. coli Genetic Stock Center
S. enterica serovar Typhimurium
DM456	ΩthiD906::MudJ(Km)	37
DM1856	ΩthiL934::Tn10d(Tc)	28