TABLE 3.
Thiamine production and expression of thiA-lacZ transcriptional fusions in wild-type and thiamine-deregulated mutants of B. subtilis
| Strain | Genotype | Accumulation (μg/liter)
|
Regulation (β-galactosidase sp act [Miller units])c
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Extracellulara | Intracellularb | Log
|
Stationary
|
||||||
| None | HMPd | TPPd | None | HMP | TPP | ||||
| TH21 | thiC+ ΩamyE::thiC-lacZ | <0.1 | 190 | 110 | 6 | 0.5 | 31 | 3 | 1.2 |
| TH22 | thiC+ ΩamyE::thiC-lacZ ΔthiL::cat4 | 0.9 | 530 | 140 | 26 | 1.3 | 75 | 9 | 1.8 |
| Tx1 | thiC+ ΩamyE::thiC-lacZ ΔthiL::cat4 thiN | 5 | 500 | NG | 135 | 8 | NG | 177 | 5 |
| Tx26 | thiC+ ΩamyE::thiC-lacZ ΔthiL::cat4 yuaJ ykoD | 15 | 500 | 280 | 35 | 130 | 120 | 20 | 36 |
a
Thiamine concentration in MM (30 ml) after 24 hours of growth at 37°C.
b
Thiamine concentration of 1-ml supernatant of sonicated cells collected from a 30-ml MM culture after 24 hours of growth at 37°C.
c
Calculated according to Miller (20). NG, no growth.
d
Final concentrations of TPP and HMP were 1 μM each.