FIG. 2.

Identification of a large polycistronic cp32-8 encoded operon by using RT-PCR. RNA was isolated and RT-PCR was performed as described in the text. Primer pairs spanning across two or more of the cp32-8 genes (indicated above each lane) were used to determine whether adjacent or nearby genes are cotranscribed. A negative control, in which RT was omitted, was performed for every primer set (only one negative control is presented in the figure). The resulting amplicons were analyzed by electrophoresis in 1.8% GTG-agarose gels. Molecular sizes markers in kilobases are indicated.