FIG. 3.

Demonstration of the transcriptional upregulation of the cp32-8 phage operon with MNNG treatment. Real-time RT-PCR was performed with RNA isolated from cells treated with or without MNNG as described in the text. (A) Ethidium bromide-stained gels of the resulting amplicons obtained from MNNG-induced (+) or uninduced cultures (−). (B) Representative real-time RT-PCR amplification curves. The thick and thin lines indicate the curves obtained with induced and uninduced cultures, respectively.