FIG. 4.

Identification of transcriptional start sites associated with the late phage operon of the cp32s. To identify transcriptional start sites for the late operon, the 5′RACE approach was used as described in the text, and the amplicons were cloned and sequenced. The origin of each sequence was determined through a BLAST search. The alignment presents the sequence determined for the upstream and 5′ domain of the family 115 paralogs, BBL42 and BBO43, the first genes of the late operon of cp32-8 and cp32-7, respectively. The transcriptional start sites (TSS), ribosome-binding sites (RBS; underlining), and translational start codons (TLS) are indicated. Identical residues are indicated by an asterisk, and the coding sequences are capitalized. The transcriptional start sites are indicated by boldface capital letters.