TABLE 1.
Primer sequences and their corresponding plasmid coordinates
| Primer | Primer sequence (5′-3′)a | Plasmid (coordinates) |
|---|---|---|
| BBL1F | CATTATCACAGGAGCTTGCTAGGC | cp32-8 (81-840) |
| BBL1R | TCCTAGTCCTTTAGCCTGCTCATT | cp32-8 (999-1022) |
| BBL4F | ACGCGGCGTTAAACTTGTTCCA | cp32-8 (2931-2952) |
| BBL4R | ACCTCCGGCCTTGATAATAACACC | cp32-8 (3133-3156) |
| BBL7F | GCTTACAAGCACAAGTTGCAGGAG | cp32-8 (4958-4981) |
| BBL7R | TGGCAGCCCATATGGTAGAAAGT | cp32-8 (5155-5177) |
| BBL11F | GGAGATGCGTCAGATGATGGACTT | cp32-8 (7079-7102) |
| BBL11R | CAGCGGCATGCCATAAGGATTTAC | cp32-8 (7310-7333) |
| BBL15F | GCTATGAGTAAAGTTGGAGGAGGC | cp32-8 (9418-9441) |
| BBL15R | GCTGCTCATAGCACCACTGGATTT | cp32-8 (9700-9723) |
| BBL16IF | AGAAAGAAATCAGGGAGATTACTCG | cp32-8 (10386-10410) |
| BBL16IR | GATGAGCGTCAAAGCGTAGCATAG | cp32-8 (10731-10754) |
| BBL17F | CAATCGCGAATTGGCAGGCAAAGA | cp32-8 (11350-11373) |
| BBL17R | GCCCATAGTCTTCTAATGCCTCGT | cp32-8 (11634-11657) |
| BBL18R | CGGTTGCTATTTTTAAGCTTGATTCG | cp32-8 (12402-12427) |
| BBL20IF | AGCACAACTTACAGTGCGGTTC | cp32-8 (13079-13100) |
| BBL20IR | TGTCTCCCTCAAGTAAAGTACCACT | cp32-8 (13274-13298) |
| BBL21IF | GCACTGACGAGTCATTTAGACTGAT | cp32-8 (13968-13992) |
| BBL21IR | GATGCAGCATATCCCGGCTTCTTT | cp32-8 (14149-14172) |
| BBL22IF | TGCCGAAGAACTTGCAGCTGAT | cp32-8 (14548-14569) |
| BBL22IR | GCTGCCATAAGCAAGATTTGATGGT | cp32-8 (14779-14803) |
| BBL24IF | TGTTGAGCTTGGACTTACGTCTT | cp32-8 (15442-15464) |
| BBL24IR | CATTTAATGGAGCGAGTGCCGCAT | cp32-8 (15621-15644) |
| BBL28F | ATGCTAAACGGCTGTAATTCTAATGAT | cp32-8 (17244-17270) |
| BBL28R | AATTCTCCAGCATCAGTTAAAGCGG | cp32-8 (17428-17452) |
| BBL1QF | GAGTGCTGCCCACAATGTTA | cp32-8 (197-216) |
| BBL1QR | CCAATCCCATGAAAACGGTA | cp32-8 (282-301) |
| BBL6QF | ATGCCCGAGCAGATAAAAGA | cp32-8 (4443-4462) |
| BBL6QR | AATTCGTCGCCCAGTTCTAA | cp32-8 (4533-4552) |
| BBL17QF | CCCTTAGACTTCACCGACGA | cp32-8 (11162-11181) |
| BBL17QR | ACCCGGATAATCAGTGCTCA | cp32-8 (11277-11296) |
| BBL23QF | TGATTTTTGTAACAGTGCTGGTTT | cp32-8 (15279-15302) |
| BBL23QR | TGTGATTTTTGCCATTACCA | cp32-8 (15382-15401) |
| L27QF | TGGAACAACTAATAGCACAAGATTT | cp32-8 (16618-16642) |
| L27QR | AGTGTTAAGTTCATTTTTGACACCA | cp32-8 (16742-16766) |
| L28QF | TGGAAAAGAAAATGGGGATG | cp32-8 (17531-17550) |
| L28QR | TAGTTGCATTGCTTGCACCA | cp32-8 (17612-17631) |
| L29QF | AAAGCACAATTTGGAGCTTGA | cp32-8 (18499-18519) |
| L29QR | GGAATTCGCGTATCAAAAGAA | cp32-8 (18385-18405) |
| L35QF | GCGTAGAAAAGAGTTTGCAAAAAG | cp32-8 (22507-22530) |
| L35QR | TGGACCTAGAATTGCTGCTG | cp32-8 (22627-22646) |
| L38QF | TGACCCCAAAAGCTATCAAAA | cp32-8 (25762-25782) |
| L38QR | CCCCTACACCCACTTATTGC | cp32-8 (25674-25693) |
| L39QF | TGATGAGCAAAGCAATGGAG | cp32-8 (26459-26478) |
| L39QR | AAATCTCCTAAGTCTGCCCAGTT | cp32-8 (26547-26569) |
| L42QF | CAAAAGTGGGAAAGTGGAGAA | cp32-8 (29090-29110) |
| L42QR | GCTCTACATCCAATTCGCTTC | cp32-8 (29211-29231) |
| L43QF | AGGGGAAGTAATTCGGCACT | cp32-8 (30010-30029) |
| L43QR | TAGTTTCTTGCCCGCATCTT | cp32-8 (30093-30112) |
| Fla F | TTCATGTTGGAGCAAACCAA | Flagellin (524-543) |
| Fla R | CTGAGCAGTTTGAGCTCCCT | Flagellin (602-621) |
| L42P1 | AGACCGCTCTACATCCAA | cp32-8 (29219-29236) |
| L42P2 | CCGTTAACAACACTTTCTCCACTTTC | cp32-8 (29099-29124) |
| L42P3 | GTTAACAACACTTTCTCCACTTTCCCA | cp32-8 (29096-29122) |
| AAP | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG | Abridged anchor primer |
| AUAP | GGCCACGCGTCGACTAGTAC | Abridged universal primer |
| L1EKF | GACGACGACAAGATGTGTGATTTAAGAAAAAC AAAACTA | cp32-8 (67-92) |
| L1EKR | GAGGAGAAGCCCGGTCAAAATGAGAATAATTTCTCTTTTAA | cp32-8 (1263-1289) |
| L6EKF | GACGACGACAAGATGGAATTATTTGATGAAAATTATTATG | cp32-8 (3963-3990) |
| L6EKR | GAGGAGAAGCCCGGTTAACTTTGCTTTATGTTAACTTGCA | cp32-8 (4897-4922) |
| L11EKF | GACGACGACAAGATGCCGCAAGATACAATTAGTGTA | cp32-8 (6702-6724) |
| L11EKR | GAGGAGAAGCCCGGTTAAGCACTTAAGCTGTTTTGATAATC | cp32-8 (7787-7813) |
| L17EKF | GACGACGACAAGATGCTGTTACTACAATATGATTTTAA | cp32-8 (10952-10977) |
| L17EKR | GAGGAGAAGCCCGGTTATTTTTTACGTTTTGTATTAGAATC | cp32-8 (11876-11902) |
| L23EKF | GACGACGACAAGATGGATACTATTAAATTAACCGAACT | cp32-8 (15215-15240) |
| L23EKR | GAGGAGAAGCCCGGTTAATCTCTTTTTTTAATGTGATTTTTG | cp32-8 (15391-15418) |
a
Tail sequences that allow ligase-independent cloning into the pET32Ek/LIC vector are indicated by underscoring.